IL12A & IL12B Protein (C-His, Rhesus)
Product Specifications
Abbreviation
IL12A & IL12B
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Bioactivity
1.Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.2-2ng/mL. 2.Immobilized Recombinant Cynomolgus IL12RB2 Protein (mFc Tag) (Cat: 90981-C38H) at 2 μg/mL (100 μL/well) can bind Recombinant Rhesus IL-12 (IL12A & IL12B Heterodimer) Protein (Cat: CT045-C08H), the EC50 is 250-750 ng/mL (QC tested) . 3.Immobilized Recombinant Rhesus IL-12 (IL12A & IL12B Heterodimer) Protein (Cat: CT045-C08H) at 0.5 μg/mL (100 μL/well) can bind Anti-IL-12 & IL-23 (P40) (Research Grade Ustekin*mab Biosimilar), the EC50 is 1.0-3.0 ng/mL (Routinely tested) .
Function
Met1-Ser219 & Met1-Ser328
Molecular Weight
The recombinant rhesus IL12A & the rhesus IL12B comprises 525 (208+317) amino acids and has a calculated molecular mass of 60.2 (24+36.2) KDa.
Species
Rhesus
Curated Selection
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