IL12A & IL12B Protein (C-human IgG1-Fc, Mouse)

Product Specifications

Abbreviation

IL12A & IL12B

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 85 % as determined by SDS-PAGE

Bioactivity

1. Immobilized Mouse IL12RB2 (His Tag) (Cat:50099-M08H) at 2 μg/mL (100 μL/well) can bind Mouse IL-12 (hFc Tag) (Cat: CT021-M02H), the EC50 is 30-340 ng/mL. 2. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.1-0.8 μg/mL.

Function

Met1-Ala215 & Met1-Ser335

Molecular Weight

The recombinant mouse IL12 heterodimer of IL12A/IL12B, both was Fc chimera, comprises 988 (434 + 554) amino acids and has a calculated molecular mass of 111.5 (48.7 + 62.8) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 55 & 75 kDa respectively in SDS-PAGE under reducing conditions.