IL12A & IL12B Protein (C-human IgG1-Fc & C-His, Mouse)

1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL12RB2-His at 10 μg/ml (100 μl/well) can bind Mouse IL12A & IL12B Heterodimer Protein (Cat:CT024-M0208H) . The EC50 of Mouse IL12A & IL12B Heterodimer Protein (Cat:CT024-M0208H) is 50.2-117.2 ng/ml. 2.Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 1-5ng/mL.

The recombinant mouse IL12 heterodimer of IL12A/IL12B, IL12A is Fc chimera, comprises 758 (434 +324) amino acids and has a calculated molecular mass of 85.9 (48.7 + 37.2) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 55 & 45 kDa respectively in SDS-PAGE under reducing conditions.