IL12A & IL12B Protein (C-His, Mouse)

Product Specifications

Abbreviation

IL12A & IL12B

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

1. Measured by its ability to bind biotinylated recombinant mouse IL12RB2 in a functional ELISA. 2. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 2-10 ng/mL.

Function

Met1-Ala215 & Met1-Ser335

Molecular Weight

The recombinant mouse IL12 heterodimer of IL12A/IL12B, comprises 528 (204 + 324) amino acids and has a calculated molecular mass of 60.3 (23.1 + 37.2) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 28 & 47 kDa respectively in SDS-PAGE under reducing conditions.