IL12A & IL12B Protein (C-His, Mouse)
Product Specifications
Abbreviation
IL12A & IL12B
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 90 % as determined by SDS-PAGE
Bioactivity
1. Measured by its ability to bind biotinylated recombinant mouse IL12RB2 in a functional ELISA. 2. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 2-10 ng/mL.
Function
Met1-Ala215 & Met1-Ser335
Molecular Weight
The recombinant mouse IL12 heterodimer of IL12A/IL12B, comprises 528 (204 + 324) amino acids and has a calculated molecular mass of 60.3 (23.1 + 37.2) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 28 & 47 kDa respectively in SDS-PAGE under reducing conditions.
Species
Mouse
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