IL12A & IL12B Protein (C-His & C-human IgG1-Fc, Mouse)

Product Specifications

Abbreviation

IL12A & IL12B

Host

HEK 293

Tag

C-His & C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 85 % as determined by SDS-PAGE

Bioactivity

1. Immobilized Recombinant Mouse IL12RB2 / IL12R-beta 2 Protein (His Tag) (Cat: 50099-M08H) at 5 μg/mL (100 μL/well) can bind Recombinant Mouse IL-12 (IL12A & IL12B Heterodimer) Protein (Cat: CT023-M0802H), the EC50 is 10-30 ng/mL (QC tested) . 2. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 15-90 ng/mL.

Function

Met1-Ala215 & Met1-Ser335

Molecular Weight

The recombinant mouse IL12 heterodimer of IL12A/IL12B, IL12B is Fc chimera, comprises 758 (204 + 554) amino acids and has a calculated molecular mass of 85.9 (23.1 + 62.8) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 28 & 75 kDa respectively in SDS-PAGE under reducing conditions.