IL10RA Protein (C-human IgG1-Fc, Rhesus)
Product Specifications
Abbreviation
IL10RA
Host
HEK 293
Tag
C-human IgG1-Fc
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Bioactivity
1. Measured by its binding ability in a functional ELISA. Immobilized Rhesus IL10RA-Fc (cat:90125-C02H) at 10 μg/ml (100 μl/well) can bind biotinylated human IL10 (Cat:10947-H07H), The EC50 of biotinylated human IL10 (Cat:10947-H07H) is 39.0-92.0 ng/ml. 2. Measured by its ability to inhibit IL-10 dependent proliferation of MC/9 mouse mast cells. Approximately 0.1-0.4 μg/mL of IL-10Rα will inhibit 50% of the biological response induced by 10 ng/mL of recombinant human IL-10.
Function
Thr9-Asn245
Molecular Weight
The recombinant rhesus IL10RA comprises 458 amino acids and has a calculated molecular mass of 51.8 KDa.
Species
Rhesus
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