IL10 Protein (N-His, Human)

Product Specifications

Abbreviation

IL10

Synonyms

CSIF, GVHDS, IL-10, IL10A, TGIF

Host

HEK 293

Tag

N-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 85 % as determined by SDS-PAGE

Bioactivity

1. Immobilized human IL10-His at 10 μg/ml (100 μl/well) can bind Cynomolgus IL10RA-Fc (Cat:90125-C02H), The EC50 of Cynomolgus IL10RA-Fc (Cat:90125-C02H) is 0.31-0.71 μg/ml. 2. Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is typically 5-20 ng/mL.

Function

Ser19-Asn178

Molecular Weight

The recombinant human IL10 comprises 176 amino acids and has a predicted molecular mass of 20.9 kDa as estimated in SDS-PAGE under reducing conditions.