IL10 Protein (Native, Human)

Product Specifications

Abbreviation

IL10

Synonyms

CSIF, GVHDS, IL-10, IL10A, TGIF

Host

E. coli

Tag

Native

Endotoxin

< 20 EU per mg protein.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1. Immobilized Recombinant Human IL10 / Interleukin-10 Protein (Cat: 10947-HNAE) at 2 μg/mL (100 μL/well) can bind Recombinant Rhesus IL10RA Protein (Fc Tag) (Cat: 90125-C02H), the EC50 is 100-300 ng/mL. 2. Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is typically 1-8 ng/mL.

Function

Ser19-Asn178

Molecular Weight

The recombinant human IL10 consists of 161 amino acids and predicts a molecular mass of 18.78 KDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions.