IGFBP4 Protein (C-His, Human)

Product Specifications

Abbreviation

IGFBP4

Synonyms

BP-4, HT29-IGFBP, IBP4, IGFBP-4

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

1. Measured by its ability to bind human IGF1 (Cat:10598-H24E) in functional ELISA. 2. Measured by its ability to bind human IGF2 (Cat:13032-H24E) in functional ELISA. 3. Measured by its ability to inhibit the biological activity of IGFI or IGFII on MCF7 human breast adenocarcinoma cells. The ED50 for this effect is typically 10-80 ng/mL in the presence of 14 ng/mL human IGFII.

Function

Met1-Glu258

Molecular Weight

The secreted recombinant human IGFBP4 comprises 248 amino acids with a predicted molecular mass of 27.4 kDa. As a result of glycosylation, it migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions.