IGFBP3 Protein (N-His, Cynomolgus)

Product Specifications

Abbreviation

IGFBP3

Host

HEK 293

Tag

N-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to inhibit the biological activity of IGFII on MCF7 human breast adenocarcinoma cells. The ED50 for this effect is typically 25-150 ng/mL in the presence of 14 ng/mL human IGFII.

Function

Gly28-Lys291

Molecular Weight

The recombinant heterodimer of cynomolgus IGFBP3 comprises 284 amino acids and has a calculated molecular mass of 31.1 KDa. The apparent molecular mass of the protein is approximately 41-44 KDa respectively in SDS-PAGE.