IGF1 Protein (N-human IgG1-Fc, Human)
Product Specifications
Abbreviation
IGF1
Synonyms
IBP1, IGF, IGF-I, IGF1A, IGFI, MGF
Host
HEK 293
Tag
N-human IgG1-Fc
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Bioactivity
1.Immobilized Recombinant Human IGF1 Protein (Fc Tag) (Cat: 10598-H01H1) at 2 μg/mL (100 μL/well) can bind Recombinant Human IGF1R Protein (ECD, Fc & AVI Tag), Biotinylated (Cat: 10164-H41H-B), the EC50 is 70-220 ng/mL. 2.Loaded Biotinylated Recombinant Human IGF1R Protein, hFc & AVI Tag (Cat. No. 10164-H41H-B) on SA Biosensor, can bind Recombinant Human IGF1 Protein, hFc Tag (Cat. No. 10598-H01H1) with an affinity constant of 8.65 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .
Function
Gly49-Ala118
Molecular Weight
The recombinant Human IGF1 consists of 330 amino acids and predicts a molecular mass of 36.1 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 36.5 kDa protein in SDS-PAGE under reducing conditions.
Species
Human
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