IGF1 Protein (Native, Human)

1.Immobilized Recombinant Human IGF1 / IGF-I Protein (Cat: 10598-HNAE) at 2 μg/mL (100 μL/well) can bind Recombinant Human IGFBP4 Protein (His Tag) (Cat: 10967-H08H), the EC50 is 40-130 ng/mL. 2. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 3.5-14 ng/mL. 3.Human breast cancer organoids were cultured with FGF7 (Cat#10210-H07E), RSPO1 (Cat#11083-HNAS), IGF1 (Cat#10598-HNAE), EGF (Cat#50482-MNCH), NRG1 Beta 1 (Cat#11609-H01H), NOG (Cat#50688-M02H) . (Routinely tested) . Data provided by D1 Medical Technology. 4.Human breast organoids were cultured with IGF1 (Cat#10598-HNAE), NRG1 Beta 1 (Cat#11609-H01H) . (Routinely tested) . Data provided by D1 Medical Technology. 5.Human ovarian organoids were cultured with IGF1 (Cat#10598-HNAE), NRG1 Beta 1 (Cat#11609-H01H), RSPO1 (Cat#11083-HNAS), EGF (Cat#50482-MNCH), NOG (Cat#50688-M02H) . (Routinely tested) . Data provided by D1 Medical Technology. 6.Western blot analysis of extracts from serum-starved MCF7, untreated (line A) ; treated with IGF-1 (Cat# 10598-HNAE) (100 ng/mL, 30 min; +) (line B) ; treated with IGF-1 and lambda-phosphatase (line C) using Phospho-S6K1 (Thr389) rabbit monoclonal Antibody (Cat# 110396-R0001) at 1:5000 dilution (upper), or Anti-RPS6KB1 rabbit polyclonal antibody (Cat# 10099-T38) (lower) at 1:5000 dilution (Routinely tested) .