IFNGR1 Protein (C-human IgG1-Fc, Rat)

1. Measured by its binding ability in a functional ELISA. Immobilized Rat IFNGR1 hFc (Cat:80182-R02H) at 2 μg/ml (100 μl/well) can bind Rat Interferon Gamma hFc (Cat:80234-R02H), Biotinylated the EC50 of Rat Interferon Gamma hFc, Biotinylated is 60-300 ng/mL. 2. Measured by its ability to inhibit rat IFNγ-mediated protection of L929 cells infected with vesicular stomatitisvirus (VSV) . The ED50 for this effect is typically 0.5-4 ng/mL in the presence of 10 ng/mL of recombinant rat IFN-gamma.

The recombinant rat IFNGR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 466 amino acids and has a predicted molecular mass of 52.4 kDa. The apparent molecular mass of the protein is approximately 117 and 65-80 kDa in SDS-PAGE under reducing conditions.