IFNG Protein (C-human IgG1-Fc, Mouse)

Product Specifications

Abbreviation

IFNG

Synonyms

If2f, Ifg, IFN-g, Ifng

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 92 % as determined by SDS-PAGE

Bioactivity

1. Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV) . The ED50 for this effect is 2.5-15 ng/mL. 2. Measured by its ability to bind with recombinant mouse IFNGR1-His (Cat:50705-M08H) in a functional ELISA.

Function

Met1-Cys155

Molecular Weight

The secreted recombinant mouse IFNG/Fc is a disulfide-linked homodimer. The reduced monomer comprises 374 amino acids and has a calculated molecular mass of 42.5 kDa. As a result of glycosylation, the apparent molecular mass of the monomer is approximately 55 kDa in SDS-PAGE under reducing conditions.