IFNG Protein (C-His, Mouse)

1. Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV) . The ED50 for this effect is typically 0.2-1 ng/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized mouse IFNG-His (cat:50709-M08H) at 10 μg/ml (100 μl/well) can bind mouse IFNGR1-Fc (Cat:50705-M02H) . The EC50 of mouse IFNGR1-Fc (Cat:50705-M02H) is 58.2-135.9 ng/ml.

The recombinant mouse IFNG comprises 144 amino acids and has a calculated molecular mass of 17 kDa. As a result of glycosylation, the apparent molecular mass of rn IFNG is approximately 21 kDa in SDS-PAGE under reducing conditions.