ICAM2 Protein (C-human IgG1-Fc, Mouse)

Product Specifications

Abbreviation

ICAM2

Synonyms

CD102, Icam-2, Icam2, Ly-60

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 93 % as determined by SDS-PAGE

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells . When cells are added to mouse ICAM2 coated plates (12.5 μg/ml, 100 μl/well), approximately 25-40% will adhere specifically.

Function

Met1-Gln222

Molecular Weight

The secreted recombinant mouse ICAM2/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 444 amino acids and has a predicted molecular mass of 50 kDa. As a result of glycosylation, the apparent molecular mass of rm ICAM2/Fc monomer is approximately 60-70 kDa in SDS-PAGE under reducing conditions.