ICAM1 Protein (C-His, Rat)

Product Specifications

Abbreviation

ICAM1

Synonyms

CD54, ICAM, Icam-1, Icam1

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 97 % as determined by SDS-PAGE

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to rat ICAM1 coated plates (12.5 μg/mL, 100 μL/well), approximately > 30% cells will adhere specifically.

Function

Met1-Thr493

Molecular Weight

The recombinant rat ICAM1 comprises 477 amino acids and predicts a molecular mass of 52.7 kDa. The apparent molecular mass of the rat ICAM1 is approximately 70-75 kDa in SDS-PAGE under reducing conditions due to glycosylation.