ICAM1 Protein (C-His, Human)

Product Specifications

Abbreviation

ICAM1

Synonyms

BB2, CD54, P3.58

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 98 % as determined by SDS-PAGE ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM1-coated plates (12.5 μg/ml, 100 μl/well), approximately 30%-60% will adhere specifically.

Function

Met1-Glu480

Molecular Weight

The recombinant human ICAM1 consists of 464 amino acids and has a predicted molecular mass of 51 kDa. As a result of glycosylation, the apparent molecular mass of rhICAM1 is approxiamtely 65-70 kDa in SDS-PAGE under reducing conditions.