HTRA2 Protein (C-His, Human)

Protease activity demonstrated by HtrA2 cleavage of bovine β-casein (Sigma, Catalog # C-6905) . Incubation of β-casein at 0.2 mg/mL with Recombinant Human HTRA-2 at 0.02 mg/mL (ratio of 10:1) for 60 minutes at 45°C in 50 mM Tris, pH 8.0, which results in >95% cleavage of β-casein, as revealed by SDS-PAGE.

The recombinant human HTRA2 consisting of 336 amino acids and has a calculated molecular mass of 36.5 kDa as estimated in SDS-PAGE under reducing conditions.