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HMPV-F Protein (C-His-AVI, HMPV)

Product Specifications

Abbreviation

HMPV-F

Host

HEK 293

Tag

C-His-AVI

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS.

Bioactivity

1.Immobilized Recombinant HMPV (CAN97-83) Fusion Protein (His & AVI Tag), Biotinylated (Cat: 40993-V49H1-B) at 1 μg/mL (100 μl/well) can bind Anti-HMPV-F (Research Grade Clone SAN32-2), the EC50 is 0.6-1.8 ng/mL (QC tested) . 2.Immobilized Recombinant HMPV (CAN97-83) Fusion Protein (His & AVI Tag), Biotinylated (Cat: 40993-V49H1-B) at 1 μg/mL (100 μl/well) can bind Anti-HMPV-F (Research Grade Clone MPV458), the EC50 is 1.1-3.3 ng/mL (Routinely tested) . 3.Immobilized Anti-HMPV Antibody (MPE8) at 2 μg/mL (100 μl/well) can bind Recombinant HMPV (CAN97-83) Fusion Protein (His & AVI Tag), Biotinylated (Cat: 40993-V49H1-B), the EC50 is 7.5-21.5 ng/mL (Routinely tested) . 4.Immobilized Anti-HMPV Antibody (SAN27-14) at 2 μg/mL (100 μl/well) can bind Recombinant HMPV (CAN97-83) Fusion Protein (His & AVI Tag), Biotinylated (Cat: 40993-V49H1-B), the EC50 is 3.5-10.5 ng/mL (Routinely tested) .

Molecular Weight

The recombinant Human metapneumovirus (HMPV) (strain CAN97-83) Fusion glycoprotein comprises 538 amino acids and has a predicted molecular mass of 58.96 kDa. It migrates as an approximately 64.7,55.2 and 16.7 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 226.5 kDa verified by SEC-MALS.

Species

HMPV

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