HMGB1 Protein (N-human IgG1-Fc, Human)

Product Specifications

Abbreviation

HMGB1

Synonyms

DKFZp686A04236, HMG-1, HMG1, HMG3, SBP-1

Host

HEK 293

Tag

N-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 97 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Loaded Biotinylated Recombinant Human RAGE Protein, hFc Tag (Cat. No. 11629-H41H-B) on SA Biosensor, can bind Recombinant Human HMGB1 Protein, hFc Tag (Cat. No. 10326-H01H) with an affinity constant of 0.307 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-Glu215

Molecular Weight

The recombinant human HMGB1/Fc is a disulfide-linked homodimeric protein.The reduced monomer consists of 452 amino acids and has a predictedmolecular mass of 51.5 kDa. As a result of glycosylation, the apparent molecular mass of rhHMGB1/Fc monomer is approximately 55-60 kDa inSDS-PAGE under reducing conditions.