HMGB1 Protein (C-His, Human)

Product Specifications

Abbreviation

HMGB1

Synonyms

DKFZp686A04236, HMG-1, HMG1, HMG3, SBP-1

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Immobilized Recombinant Human HMGB1 Protein (His Tag) (Cat: 10326-H08H1) at 2 μg/mL (100 μL/well) can bind Recombinant Human AGER / RAGE Protein (Fc Tag) (Cat: 11629-H02H), the EC50 is 40-120 ng/mL.

Function

Gly2-Glu215

Molecular Weight

The recombinant Human HMGB1 consisting of 225 amino acids and has a calculated molecular mass of 26.21 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 33.2 kDa protein in SDS-PAGE under reducing conditions.