HGF Protein (Native, Human)

Abbreviation

HGF

Synonyms

DFNB39, F-TCF, HGFB, HPTA, SF

Host

CHO

Tag

Native

Endotoxin

< 0.01 EU per μg of the protein.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Measured by its ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell proliferation. The ED50 for this effect is typically 0.2-2 ng/mL. 2.Immobilized Recombinant Human HGF / Hepatocyte Growth Factor Protein (Cat: 10463-HNAS) at 2μg/mL (100μL/well) can bind Recombinant Human c-MET / HGFR Protein (His Tag) (Cat: 10692-H08H), the EC50 is 16-48 ng/mL. 3.Human cholangiocarcinomas organoids were cultured with FGF2 (Cat#10014-HNAE), HGF (Cat#10463-HNAS), FGF7 (Cat#10210-H07E), EGF (Cat#50482-MNCH), FGF10 (Cat#10573-HNAE), NOG (Cat#50688-M02H), RSPO1 (Cat#11083-HNAS) . (Routinely tested) . Data provided by D1 Medical Technology. 4.Human liver cancer organoids were cultured with FGF2 (Cat#10014-HNAE), HGF (Cat#10463-HNAS), FGF7 (Cat#10210-H07E), EGF (Cat#50482-MNCH), FGF10 (Cat#10573-HNAE), TGFB1 (Cat#10804-HNAC), NOG (Cat#50688-M02H), RSPO1 (Cat#11083-HNAS) . (Routinely tested) . Data provided by D1 Medical Technology. 5.Human kidney organoids were cultured with FGF7 (Cat#10210-H07E), EGF (Cat#50482-MNCH), FGF10 (Cat#10573-HNAE), NOG (Cat#50688-M02H), RSPO1 (Cat#11083-HNAS), HGF (Cat#10463-HNAS), FGF4 (Cat#16043-HNAE) . (Routinely tested) . Data provided by D1 Medical Technology. 6.Western blot analysis of extracts from A549, untreated (line A) or treated with HGF (Cat:10463-HNAS) (50 ng/ml, 5min; +) (line B), using Phospho-Met (Tyr1234, 1235) Rabbit monoclonal Antibody (Cat# 110587-R0060) at 1:1000 dilution (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal (Cat: 100166-MCR10) at 1:50000 dilution (lower) (Routinely tested) .

Function

Met1-Ser728

Molecular Weight

The secreted recombinant human HGF consists of 697 amino acids after cleavage of the signal peptide and has a predicted molecular mass of 79.7 kDa. The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain. As a result of glycosylation, it migrates with the apparent molecular mass of 90, 60 and 34 kDa corresponding to the single chain, α chain and β chain respectively in SDS-PAGE under reducing conditions.

Species

Human