GZMB Protein (C-His, Human)

Product Specifications

Abbreviation

GZMB

Synonyms

C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, GRB, HLP, SECT

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 97 % as determined by SDS-PAGE ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to cleave a peptide substrate, tButyloxycaronylAlaAlaAspThioBenzylester (BocAADSBzl), in the presence of 5,5’Dithiobis (2nitrobenzoic acid) (DTNB) . (The enzyme needs to be activated by Recombinant Mouse Active Cathepsin C) .

Function

Met1-Tyr247

Molecular Weight

The recombinant human Granzyme B consists of 240 amino acids and has a calculated molecular mass of 27.2 kDa. As a result of glycosylation, the apparent molecular mass of rhGranzyme B is approximately 35-40 kDa in SDS-PAGE under reducing conditions.