GPRC5D Protein (N-Flag & C-His, Cynomolgus, Rhesus)
Product Specifications
Abbreviation
GPRC5D
Host
HEK 293
Tag
N-Flag & C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
≥ 80 % as determined by SDS-PAGE.
Bioactivity
1.Immobilized Recombinant Rhesus GPRC5D-Nanodisc Protein (His & Flag Tag) (Cat: 91022-C18H-NA) at 5 μg/mL (100 μL/well) can bind Anti-Rhesus GPRC5D Antibody, the EC50 is 17.5-52.5 ng/mL (QC tested) . 2.Anti-Rhesus GPRC5D Antibody captured on Protein A chip, can bind Recombinant Cynomolgus / Rhesus GPRC5D Protein (Nanodisc, His & Flag Tag) (Cat: 91022-C18H-NA) with an affinity constant of 0.475 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3.Anti-Human GPRC5D Monoclonal Antibody, Human IgG1 captured on CM5 chip via Anti-Human IgG Fc, can bind Recombinant Cynomolgus / Rhesus GPRC5D Protein (Nanodisc, His & Flag Tag) (Cat: 91022-C18H-NA) with an affinity constant of 0.354 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .
Function
Met1-Ser268
Molecular Weight
The recombinant Cynomolgus / Rhesus GPRC5D consists of 286 amino acids and predicts a molecular mass of 32.95 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 31.7 kDa protein in SDS-PAGE under reducing conditions.
Species
Cynomolgus, Rhesus
Curated Selection
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