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GPRC5D Protein (N-Flag & C-His, Cynomolgus, Rhesus)

Product Specifications

Abbreviation

GPRC5D

Host

HEK 293

Tag

N-Flag & C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 80 % as determined by SDS-PAGE.

Bioactivity

1.Immobilized Recombinant Rhesus GPRC5D-Nanodisc Protein (His & Flag Tag) (Cat: 91022-C18H-NA) at 5 μg/mL (100 μL/well) can bind Anti-Rhesus GPRC5D Antibody, the EC50 is 17.5-52.5 ng/mL (QC tested) . 2.Anti-Rhesus GPRC5D Antibody captured on Protein A chip, can bind Recombinant Cynomolgus / Rhesus GPRC5D Protein (Nanodisc, His & Flag Tag) (Cat: 91022-C18H-NA) with an affinity constant of 0.475 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3.Anti-Human GPRC5D Monoclonal Antibody, Human IgG1 captured on CM5 chip via Anti-Human IgG Fc, can bind Recombinant Cynomolgus / Rhesus GPRC5D Protein (Nanodisc, His & Flag Tag) (Cat: 91022-C18H-NA) with an affinity constant of 0.354 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .

Function

Met1-Ser268

Molecular Weight

The recombinant Cynomolgus / Rhesus GPRC5D consists of 286 amino acids and predicts a molecular mass of 32.95 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 31.7 kDa protein in SDS-PAGE under reducing conditions.

Species

Cynomolgus, Rhesus

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