GPC3 Protein (C-His, Human)

1.Flow cytometric analysis of anti-GPC3 CAR expression. 5e5 of anti-GPC3 CAR-293 cells were stained with 100 μL of 1:100 (1 μL stock solution in 100 μL FACS buffer) of PE-conjugated human GPC3 Protein (Site-Specific PE Conjugation, His Tag, Cat. No. 10088-H86H-SP) and negative control protein. Non-transduced 293 cells were used as a control (left) . PE signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of PE-conjugated GPC3 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and PE-conjugated human GPC3 Protein (Site-Specific PE Conjugation, His Tag, Cat. No. 10088-H86H-SP) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. PE signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) .

The recombinant human GPC3 comprises 546 amino acids and has a predicted molecular mass of 64 kDa, Cleaved partially by a furin-like convertase to N and C-terminal fragment with predicted molecular mass of 38 KDa and 26 kDa.