Gp140 Protein (C-His, HIV)

1.Immobilized Recombinant HIV-1 gp140 Protein (group M, subtype CRF07_BC) (His Tag) (Cat: 11677-V08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD4 Protein (Fc Tag) (Cat: 10400-H02H), the EC50 is 70-210 ng/mL. 2.Loaded Recombinant Human CD4 Protein, hFc Tag (Cat. No. 10400-H02H) on ProA Biosensor, can bind HIV-1 gp140 Protein (group M, subtype CRF07_BC), His Tag (Cat. No. 11677-V08H) with an affinity constant of 82.3 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant HIV-1 gp140 consists of 694 amino acids after removal of the signal peptide and has a predicted molecular mass of 78.2 kDa. The apparent molecular mass of the recombinant protein is approximately 119 KDa in SDS-PAGE under reducing conditions.