Gp140 Protein (C-human IgG1-Fc, HIV)

Product Specifications

Abbreviation

Gp140

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE

Bioactivity

Using the Octet RED System, the affinity constant (Kd) of gp140-Fc bound to human CD4 is 0.3 μM.

Function

Ala29-Ser661

Molecular Weight

The recombinant HIV-1 gp140 consists of 874 amino acids after removal of the signal peptide and has a predicted molecular mass of 98 kDa. The apparent molecular mass of the recombinant protein is approximately 210-230 kDa in SDS-PAGE under reducing conditions.