GHR Protein (C-human IgG1-Fc & His, Mouse)

Measured by its ability to Inhibit activation of GH pathway in a Luciferase receptor Assay System. The ED50 for this effect is typically 0.1-1 μg/mL in the presence of 50 ng/mL human growth hormone.

The recombinant mouse GHR/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 497 amino acids and has a predicted molecular mass of 56.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmGHR/Fc monomer is approximately 70-80 kDa due to glycosylation.