FZD5 Protein (C-His, Human)

Product Specifications

Abbreviation

FZD5

Synonyms

C2orf31, DKFZP434E2135, HFZ5, MCOPCB11

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 97 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Recombinant Human Frizzled-5 / FZD5 Protein (His Tag) (Cat: 10473-H08H) at 2μg/mL (100μL/well) can bind pan-FZD antibody, Human IgG2, the EC50 is 12-36 ng/mL. 2.Vantictumab captured on Protein A chip can bind Recombinant Human Frizzled-5 Protein, His Tag (Cat. No. 10473-H08H) with an affinity constant of 161.1 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .

Function

Met1-Pro167

Molecular Weight

The recombinant human frizzled5 consists of 152 amino acids and has a predicted molecular mass of 17.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhfrizzled5 is approximately 25-30 kDa due to glycosylation.