FLT4 Protein (C-human IgG1-Fc, Human)

1.Immobilized recombinant human VEGFR3 at 5 μg/ml (100 μl/well) can bind recombinant human VEGF-D at a linear range of 62.5-2000 ng/ml (Routinely tested) . 2.Immobilized Recombinant Human VEGF-C Protein (His Tag) (Cat: 10542-H08H) at 5 μg/mL (100 μL/well) can bind Recombinant Human VEGFR3 / FLT4 Protein (ECD, Fc Tag) (Cat: 10806-H02H), the EC50 is 3-9 ng/mL (QC tested) . 3.Recombinant Human VEGF-C Protein (His Tag) (Cat: 10542-H08H) captured on NTA chip, can bind Recombinant Human VEGFR3 / FLT4 Protein (ECD, Fc Tag) (Cat: 10806-H02H) with an affinity constant of 0.855 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

The recombinant human VEGFR3/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 990 amino acids and predicts a molecular mass of 111.3 kDa. As a result of glycosylation and proteolytic cleavage, rhVEGFR3/Fc migrates as three bands (160, 85, 75 kDa) corresponding to the full length and the cleaved two polypeptides respectively in SDS-PAGE under reducing conditions.