FLT4 Protein (C-human IgG1-Fc, Mouse)

1. Measured by its ability to bind human VEGF-D (Cat: 10557-H08H) in functional ELISA. 2. Immobilized human VEGF-C (Cat: 10542-H08H) at 10 μg/mL (100 μL/well) can bind mouse VEGFR3-Fc. The EC50 of mouse VEGFR3-Fc is 0.008 μg/mL. 3. Measured by its ability to bind with mouse FIGF-His (Cat:50157-M08H) in a functional ELISA.

The secreted recombinant mouse FLT4/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 992 amino acids and has a predicted molecular mass of 112 kDa. As a result of glycosylation and proteolytic cleavage, rm FLT4/Fc monomer migrates as three bands (150, 85, 65 kDa) corresponding to the full length and the cleaved two polypeptides respectively in SDS-PAGE under reducing conditions.