FLT1 Protein (C-human IgG1-Fc, Human)

1. Measured by its binding ability in a functional ELISA. Immobilized human VEGFR1-Fc (Cat:10136-H02H) at 10μg/mL (100μL/well) can bind biotinylated human VEGF165 (Cat:11066-HNAH), the EC50 of biotinylated human VEGF165 is 10-40 ng/mL. 2. Measured by its ability to inhibit VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC) . The ED50 for this effect is typically 20-100 ng/mL in the presence of 10 ng/mL rhVEGF165.