FLT1 Protein (C-His, Human)

1.Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC) . (Conn, G. et al. ,1990 , Proc. Natl. Acad. Sci. USA 87:1323.) The ED50 for this effect is typically 10-40 ng/mL in the presence of 10 ng/mL human VEGF165 (Routinely tested) . 2.Immobilized Recombinant Human VEGFR1 / FLT-1 Protein (His Tag) (Cat: 10136-H08H1) at 2 μg/mL (100 μL/well) can bind Recombinant Human / Cynomolgus VEGF / VEGFA / VEGF165 Protein (Cat: 11066-HNAH), the EC50 is 9.7-29.3 ng/mL (Routinely tested) . 3.Immobilized Recombinant Human / Cynomolgus VEGF / VEGFA / VEGF165 Protein (Cat: 11066-HNAH) at 0.1 μg/mL (100 μL/well) can bind Recombinant Human VEGFR1 / FLT-1 Protein (His Tag) (Cat: 10136-H08H1), the EC50 is 3-10 ng/mL (QC tested) .