FGFR4 Protein (C-human IgG1-Fc, Rat)

1. Measured by its binding ability in a functional ELISA. 2. Immobilized human FGF18 (Cat: 13206-H08H) at 10 μg/mL (100 μl/well) can bind Rat FGFR4 (Cat:80093-R02H), The EC50 of Rat FGFR4 (Cat:80093-R02H) is 1.17 μg/mL. 3. Immobilized mouse FGF18 (Cat:50177-M08H) at 10 μg/mL (100 μl/well) can bind Rat FGFR4 (Cat:80093-R02H), The EC50 of Rat FGFR4 (Cat:80093-R02H) is 0.44 μg/mL. 4. Immobilized human bFGF at 10 μg/mL (100 μl/well) can bind Rat FGFR4 (Cat:80093-R02H), The EC50 of Rat FGFR4 (Cat:80093-R02H) is 0.163 μg/mL.

The recombinant rat FGFR4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 592 amino acids and predicts a molecular mass of 66 kDa. The apparent molecular mass of the rat FGFR4/Fc monomer is approximately 110 kDa in SDS-PAGE under reducing conditions due to glycosylation.