FGFR1 Protein (C-human IgG1-Fc, Mouse)

Product Specifications

Abbreviation

FGFR1

Synonyms

BFGF-R-1, c-fgr, Eask, Fgfr-1, FGFR-I, Fgfr1, FLG, Flt-2, Fr1, Hspy, MFR

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 97 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to inhibit mouse FGF acidic (aFGF/FGF1) dependent proliferation of Balb/C 3T3 mouse fibroblasts. The ED50 for this effect is typically 6-25 ng/mL in the presence of 0.5 ng/mL of mouse FGF acidic (aFGF/FGF1) .

Function

Met1-Glu376

Molecular Weight

The secreted recombinant mouse FGFR1/Fc is a disulfide-linked homodimer. The reduced monomer consists of 596 amino acids and has a calculated molecular mass of 66.5 kDa. The apparent molecular mass of rmFGFR1/Fc monomer is approximately 100-110 kDa in SDS-PAGE under reducing conditions due to glycosylation.