FCGRT & B2M Protein (C-AVI & His, Bovine)

Product Specifications

Abbreviation

FCGRT & B2M

Host

HEK 293

Tag

C-AVI & His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

Biotinylated Recombinant Bovine FCGRT & B2M Heterodimer Protein, AVI & His Tag (Cat. No. CT151-B27H-B) captured on NTA chip, can bind Herceptin derived Anti-Erbb2 antibody, Human IgG1 with an affinity constant of 0.220 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .

Function

Met1-Pro298 & Met1-Leu118

Molecular Weight

The recombinant Bovine FCGRT & B2M Heterodimer consists of 398 (300+98) amino acids and predicts a molecular mass of 45.03 (33.56+11.47) KDa. it migrates as an approximately 35.4 and 19 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 50.0 kDa verified by SEC-MALS (Routinely tested) .