FCGRT & B2M Protein (C-AVI & His, Cynomolgus)

Product Specifications

Abbreviation

FCGRT & B2M

Host

HEK 293

Tag

C-AVI & His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE, ≥ 90 % as determined by SEC-HPLC, ≥ 90% as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Anti-CD20 Antibody at 2 μg/ml (100 μl/well) can bind Recombinant Cynomolgus FCGRT&B2M Heterodimer Protein (His&AVI Tag), Biotinylated (Cat: CT084-C27H-B), the EC50 is 80-240 ng/mL. 2.Loaded Biotinylated Cynomolgus FcRn recombinant protein (His & Avi Tag) (Cat. CT084-C27H-B) on SA Biosensor, can bind IgG1 Fc (Cat. 10702-HNAC) with an affinity constant of 0.08 μM as determined in a BLI assay (Sartorius Octet Red384) .

Function

Met 1-Ser 297 & Met 1-Met 119

Molecular Weight

The recombinant cynomolgus FCGRT&B2M consists of 399 (300+99) amino acids and predicts a molecular mass of 45.3 (33.7+ 11.6) KDa. The molecular weight of this protein is around 46-56 kDa verified by SEC-MALS (Routinely tested) .