FCGRT & B2M Protein (C-His, Sus scrofa (Pig) )
Product Specifications
Abbreviation
FCGRT & B2M
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .
Bioactivity
1.Recombinant Sus scrofa (Pig) FCGRT & B2M Heterodimer Protein, His Tag (Cat. No. CT149-W08H) captured on NTA chip, can bind Anti-CD20 (Research Grade Rituximab Biosimilar) with an affinity constant of 0.119 μM as determined in an SPR assay (Biacore T200) (QC tested) . 2.Anti-CD20 (Research Grade Rituximab Biosimilar) immobilized on CM5 chip, can bind Recombinant Porcine FCGRT & B2M Heterodimer Protein (His Tag) (Cat: CT149-W08H) with an affinity constant of 0.690 μM as determined in an SPR assay (Biacore T200) (Routinely tested) .
Function
Asp16-Ser289 & Met1-His118
Molecular Weight
The recombinant Sus scrofa (Pig) FCGRT & B2M Heterodimer comprises 383 (285+98) amino acids and has a predicted molecular mass of 43.31 (31.89+11.42) kDa. It migrates as an approximately 34.2 kDa and 13.4 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 47.8 kDa verified by SEC-MALS.
Species
Sus scrofa (Pig)
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