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FCGRT & B2M Protein (C-His, Canine)

Product Specifications

Abbreviation

FCGRT & B2M

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Recombinant Canine FCGRT & B2M Heterodimer Protein, His Tag (Cat. No. CT148-D08H) captured on NTA chip, can bind Rituximab with an affinity constant of 0.225 μM as determined in an SPR assay (Biacore T200) (Routinely tested) . 2.Recombinant Canine FCGRT & B2M Heterodimer Protein, His Tag (Cat. No. CT148-D08H) captured on CM5 chip via Anti-his antibody, can bind Herceptin with an affinity constant of 0.213 μM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3.Recombinant Canine FCGRT & B2M Heterodimer Protein, His Tag (Cat. No. CT148-D08H) captured on CM5 chip via Anti-his antibody, can bind Ipilimumab with an affinity constant of 0.252 μM as determined in an SPR assay (Biacore T200) (QC tested) . 4.Recombinant Canine FCGRT & B2M Heterodimer Protein, His Tag (Cat. No. CT148-D08H) captured on CM5 chip via Anti-his antibody, can bind mOKT3 with an affinity constant of 1.480 μM as determined in an SPR assay (Biacore T200) (Routinely tested) .

Function

Ala23-Pro298 & Met1-Asn125

Molecular Weight

The recombinant Canine FCGRT & B2M Heterodimer comprises 385 (287+98) amino acids and has a predicted molecular mass of 43.41 (31.89+11.52) kDa. It migrates as an approximately 33.2 kDa and 12.7 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 47.4 kDa verified by SEC-MALS.

Species

Canine

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