FCGRT & B2M Protein (C-AVI & His, Canine)
Product Specifications
Abbreviation
FCGRT & B2M
Host
HEK 293
Tag
C-AVI & His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS (Routinely tested) .
Bioactivity
1.Biotinylated Recombinant Canine FCGRT & B2M Heterodimer Protein, AVI & His Tag (Cat. No. CT148-D27H-B) captured on NTA chip, can bind Anti-CD20 (Research Grade Rituximab Biosimilar) with an affinity constant of 0.211 μM as determined in an SPR assay (Biacore T200) (Routinely tested) . 2.Recombinant Canine FCGRT & B2M Heterodimer Protein (AVI & His Tag), Biotinylated (Cat: CT148-D27H-B) captured on SA chip, can bind anti-ERBB2 (Research grade trastuz*mab biosimilar) with an affinity constant of 0.154 μM as determined in an SPR assay (Biacore 8K) (Routinely tested) .
Function
Ala23-Pro298 & Met1-Asn125
Molecular Weight
The recombinant Canine FCGRT & B2M Heterodimer comprises 400 (302+98) amino acids and has a predicted molecular mass of 45.22 (33.7+11.52) kDa. It migrates as an approximately 36.4 and 13.1 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 48.7 kDa verified by SEC-MALS (Routinely tested) .
Species
Canine
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