FCGR4 Protein (C-His, Mouse)

1.Loaded Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (OKT3) on ProA Biosensor, can bind Recombinant Mouse FCGR4 Protein, His Tag (Cat. No. 50036-M08H) with an affinity constant of 3.43nM as determined in BLI assay (Sartorius Octet RED384) (QC tested) . 2.Loaded Recombinant Mouse FCGR4 Protein, His Tag (Cat. No. 50036-M08H) on His1K Biosensor, can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (OKT3) with an affinity constant of 17.7nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Loaded Recombinant Mouse CD16-2/FCGR4/Fc gamma RIV Protein, His Tag (Cat. No. 50036-M08H) on NTA Biosensor, can bind Herceptin derived Anti-Erbb2 Antibody, Human IgG1 with an affinity constant of 69.300 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Recombinant Mouse CD16-2/FCGR4/Fc gamma RIV Protein, His Tag (Cat. No. 50036-M08H) captured on CM5 chip via Anti-his antibody, can bind OKT3 derived Anti-CD3 antibody, mouse IgG2a with an affinity constant of 0.228 μM as determined in an SPR assay (Biacore T200) (Routinely tested) .

The recombinant mouse FCGR4 consists of 194 amino acids and has a predicted molecular mass of 22.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFCGR4 is approximately 25-35 kDa due to glycosylation.