FCGR2B Protein (C-AVI & His, Cynomolgus)

Product Specifications

Abbreviation

FCGR2B

Host

HEK 293

Tag

C-AVI & His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 97 % as determined by SDS-PAGE

Bioactivity

1.Immobilized Cynomolgus CD32B/Fcgr2b Protein (His & AVI Tag), Biotinylated (Cat:90014-C27H-B) at 2 μg/mL (100 μL/well) can bind Human IgG2 Protein (257 Ser/Ala) (Cat:13504-HNAH), the EC50 is 4-36 ng/mL. 2.Loaded Recombinant Cynomolgus CD32B / Fcgr2b Protein, His & AVI Tag, Avi-tag Biotinylated (Cat. No. 90014-C27H-B) on SA Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 1.4 μM as determined in BLI assay (Sartorius Octet RED384) (QC tested) .

Function

Met1-Gly220

Molecular Weight

The recombinant cynomolgus CD32b consists of 211 amino acids and has a calculated molecular mass of 23.5 kDa. The apparent molecular mass of the recombinant protein is approximately 34 kDa in SDS-PAGE under reducing conditions due to glycosylation.