FCGR2B Protein (C-AVI & His, Mouse)

Product Specifications

Abbreviation

FCGR2B

Synonyms

CD32, F630109E10Rik, Fcgr2, FcgRII, Fcr-2, Fcr-3, fcRII, Fc[g]RII, Ly-17, Ly-m20, LyM-1

Host

HEK 293

Tag

C-AVI & His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC

Bioactivity

1.Loaded Recombinant Mouse CD32B/Fcgr2b Protein, His Tag, Biotinylated (Cat. No. 50030-M27H-B) on SA Biosensor, can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (OKT3) with an affinity constant of 2.32μM as determined in BLI assay (Sartorius Octet RED384) (QC tested) . 2.Recombinant Mouse CD32 / FCGR2B Protein (His & AVI Tag), Biotinylated (Cat: 50030-M27H-B) immobilized on SA chip, can bind Anti-CD3 (Research Grade OKT3 Biosimilar) with an affinity constant of 6.980 μM as determined in an SPR assay (Biacore 8K) (QC tested) .

Function

Met1-Arg217

Molecular Weight

The secreted recombinant mouse FGGR2B consists of 212 amino acids and has a predicted molecular mass of 23.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FGGR2B is approximately 38-43 kDa due to glycosylation.