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FCGR1 Protein (C-His, Canine)

Product Specifications

Abbreviation

FCGR1

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Anti-HER2 (Research Grade Trastuzumab Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Canine CD64/FCGR1 Protein (His Tag) (Cat: 70269-D08H), the EC50 is 0.8-2.5 ng/mL (Routinely tested) . 2.Recombinant Canine CD64/FCGR1 Protein (His Tag) (Cat: 70269-D08H) captured on CM5 chip via Anti-His antibody, can bind Anti-CTLA4 (Research Grade Ipilimumab Biosimilar) with an affinity constant of 0.982 nM as determined in an SPR assay (Biacore 8K) (QC tested) . 3.Recombinant Canine CD64/FCGR1 Protein (His Tag) (Cat: 70269-D08H) captured on CM5 chip, can bind anti-ERBB2 (Research grade trastuz*mab biosimilar) with an affinity constant of 2.720 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 4.Recombinant Canine CD64/FCGR1 Protein (His Tag) (Cat: 70269-D08H) captured on CM5 chip via Anti-His antibody chip, can bind Anti-CD3 (Research Grade OKT3 Biosimilar) with an affinity constant of 0.441 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

Function

Val11-Pro288

Molecular Weight

The recombinant Canine FCGR1 comprises 284 amino acids and has a predicted molecular mass of 31.92 kDa. It migrates as approximately 43.6 kDa and 40.2 kDa band in SDS-PAGE under reducing conditions.

Species

Canine

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