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FCGR1 Protein (C-AVI & His, Human)

Product Specifications

Abbreviation

FCGR1

Host

HEK 293

Tag

C-AVI & His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE. > 85 % as determined by SEC-HPLC

Bioactivity

1.Immobilized Anti-CD20 (Research Grade Rituximab Biosimilar) at 1 μg/mL (100 μL/well) can bind Recombinant Human CD64 / FCGR1A Protein (His & AVI Tag), Biotinylated (Cat:10256-H27H-B), the EC50 1-3 ng/mL (QC tested) . 2.Loaded Biotinylated Human FcγRI / CD64 recombinant protein (His & Avi Tag) (Cat. 10256-H27H-B) on SA Biosensor, can bind Anti-PD1 (Research Grade Nivolumab Biosimilar) with an affinity constant of 6.6 nM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested) . 3.Loaded Anti-CD20 (Research Grade Rituximab Biosimilar) on ProA Biosensor, can bind Biotinylated Human FcγRI / CD64 recombinant protein (His & Avi Tag) (Cat. 10256-H27H-B) with an affinity constant of 0.54 nM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested) . 4.Loaded Biotinylated Human FcγRI / CD64 recombinant protein (His & Avi Tag) (Cat. 10256-H27H-B) on SA Biosensor, can bind Anti-VEGF (Research Grade Bevacizumab Biosimilar) with an affinity constant of 1.35 nM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested) . 5.Recombinant Human CD64 / FCGR1A Protein (His & AVI Tag), Biotinylated (Cat: 10256-H27H-B) captured on Biotin CAP-Series S Sensor chip, can bind anti-ERBB2 (Research grade trastuz*mab biosimilar) with an affinity constant of 0.926 nM as determined in an SPR assay (Biacore 8K) (QC tested) .

Function

Met1-Thr287

Molecular Weight

The secreted recombinant human CD64 consists of 307 amino acids and has a predicted molecular mass of 34.4 kDa.

Species

Human

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