FCGR1 Protein (C-His, Mouse)

1.Immobilized Anti-CD20 (Research Grade Rituximab Biosimilar) (IgG1) at 1 μg/mL can bind Mouse FcγRI / CD64 recombinant protein (Cat:50086-M08H), the EC50 is 16-35 ng/mL (Routinely tested) . 2.Immobilized Anti-CD3 (Research Grade OKT3 Biosimilar) (mouse IgG2a) at 2 μg/mL (100 μL/well) can bind Recombinant Mouse CD64 / FCGR1 Protein (His Tag) (Cat: 50086-M08H), the EC50 is 0.8-2.4 ng/mL (QC tested) . 3.Loaded Mouse FcγRI / CD64 recombinant protein (Cat:50086-M08H) on His1K Biosensor, can bind Anti-VEGF (Research Grade Bevacizumab Biosimilar) (IgG1) with an affinity constant of 0.12 μM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested) . 4.Loaded Mouse FcγRI / CD64 recombinant protein (Cat:50086-M08H) on His1K Biosensor, can bind Anti-PD1 (Research grade Nivolumab biosimilar) (IgG4) with an affinity constant of 0.28 μM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested) . 5.Captured Mouse FcγRI / CD64 recombinant protein (Cat:50086-M08H) on Anti-His Chip can bind Anti-VEGF (Research Grade Bevacizumab Biosimilar) (IgG1) with an affinity constant of 0.12 μM as determined in an SPR assay (Biacore T200) (Routinely tested) . 6.Captured Mouse FcγRI / CD64 recombinant protein (Cat:50086-M08H) on Anti-His Chip can bind Anti-PD1 (Research grade Nivolumab biosimilar) (IgG4) with an affinity constant of 0.69 μM as determined in an SPR assay (Biacore T200) (Routinely tested) . 7.Loaded Anti-CD3 (Research Grade OKT3 Biosimilar) (mouse IgG2a) on ProA Biosensor, can bind Recombinant Mouse CD64 Protein, His Tag (Cat. No. 50086-M08H) with an affinity constant of 3.46nM as determined in BLI assay (Sartorius Octet RED384) (QC tested) . 8.Loaded Recombinant Mouse CD64 Protein, His Tag (Cat. No. 50086-M08H) on His1K Biosensor, can bind Anti-CD3 (Research Grade OKT3 Biosimilar) (mouse IgG2a) with an affinity constant of 26.7nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 9.Immobilized Recombinant Mouse CD64 / FCGR1 Protein (His Tag) (Cat: 50086-M08H) at 5 μg/mL (100 μL/well) can bind Anti-CD3 (OKT3) (Research Grade Muromonab Biosimilar), Biotinylated, the EC50 is 113.4 ng/mL (Routinely tested) . 10.Loaded Anti-HER2 (Research Grade Trastuzumab Biosimilar) (Cat. No. 68046-H002) on ProA Biosensor, can bind Recombinant Mouse CD64 / FCGR1 Protein (His Tag) (Cat. No. 50086-M08H) with an affinity constant of 47.8 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant mouse FCGR1 consists of 284 amino acids after removal of the signal peptide and has a predicted molecular mass of 32 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FCGR1 is approximately 45-50 kDa due to glycosylation. The molecular weight of this protein is around 40-50 kDa verified by SEC-MALS (Routinely tested) .