FCGR1 Protein (C-AVI & His, Mouse)

Product Specifications

Abbreviation

FCGR1

Synonyms

CD64, Fcg1, FcgammaRI, Fcgr1, IGGHAFC

Host

HEK 293

Tag

C-AVI & His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE, ≥ 95 % as determined by SEC-HPLC, ≥ 95% as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Anti-CD20 (Research Grade Rituximab Biosimilar) (IgG1) at 1 μg/mL can bind Biotinylated Mouse FcγRI / CD64 recombinant protein (His & Avi Tag) (Cat. 50086-M27H-B), the EC50 is 20-50 ng/mL (QC tested) . 2.Loaded Biotinylated Mouse FcγRI / CD64 recombinant protein (His & Avi Tag) (Cat. 50086-M27H-B) on SA Biosensor, can bind Anti-VEGF (Research Grade Bevacizumab Biosimilar) (IgG1) with an affinity constant of 0.66 μM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested) . 3.Loaded Biotinylated Mouse FcγRI / CD64 recombinant protein (His & Avi Tag) (Cat. 50086-M27H-B) on SA Biosensor, can bind Anti-PD1 (Research grade Nivolumab biosimilar) (IgG4) with an affinity constant of 1.9 μM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested) . 4.Recombinant Mouse CD64 / FCGR1 Protein (His & AVI Tag), Biotinylated (Cat: 50086-M27H-B) captured on Biotin CAP-Series S Sensor chip, can bind anti-ERBB2 (Research grade trastuz*mab biosimilar) with an affinity constant of 0.106 μM as determined in an SPR assay (Biacore 8K) (QC tested) .

Function

Met1-Pro297

Molecular Weight

The recombinant mouse FCGR1 consists of 308 amino acids after removal of the signal peptide and has a predicted molecular mass of 34.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FCGR1 is approximately 50-55 kDa due to glycosylation. The molecular weight of this protein is around 41-51 kDa verified by SEC-MALS (Routinely tested) .

Species

Mouse