FCGR1 Protein (C-His, Canine)

Product Specifications

Abbreviation

FCGR1

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Trastuzumab derived Anti-Erbb2 antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Canine CD64/FCGR1 Protein (His Tag) (Cat: 70269-D08H1), the EC50 is 1.0-3.0 ng/mL (QC tested) . 2.Loaded Recombinant Canine CD64/FCGR1 Protein, His Tag (Cat. No. 70269-D08H1) on HIS1K Biosensor, can bind Herceptin derived Anti-Erbb2 Antibody, Human IgG1 with an affinity constant of 7.430 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Gln16-Pro288

Molecular Weight

The recombinant Canine FCGR1 comprises 284 amino acids and has a predicted molecular mass of 31.92 kDa. It migrates as approximately 41.2 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 39.7 kDa verified by SEC-MALS (Routinely tested) .