FCER1A Protein (C-human IgG1-Fc & AVI, Human)
Product Specifications
Abbreviation
FCER1A
Synonyms
FCE1A, FcepsilonRIalpha, FcERI, FCERIA
Host
HEK 293
Tag
C-human IgG1-Fc & AVI
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS (Routinely tested) .
Bioactivity
1.Immobilized Recombinant Human Fc epsilon RI Protein (Fc & AVI Tag), Biotinylated (Cat: 13193-H41H-B) at 2 μg/mL (100 μl/well) can bind Recombinant Human IgE-Fc Protein (Constant Domain 3&4, His Tag) (Cat:29702-H08H), the EC50 is 3-11 ng/mL (QC tested) . 2.Loaded Recombinant Human Fc epsilon RI Protein (Fc & AVI Tag), Biotinylated (Cat. No. 13193-H41H-B) on SA (Streptavidin), can bind Recombinant Human IgE-Fc Protein (Constant Domain 3&4, His Tag) (Cat. No. 29702-H08H) with an affinity constant of 4.07 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .
Function
Met1-Gln205
Molecular Weight
The recombinant Human FCER1A consists of 433 amino acids and predicts a molecular mass of 49.57 kDa. It migrates as approximately 85.7 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 140.7 kDa verified by SEC-MALS (Routinely tested) .
Species
Human
Curated Selection
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